1,3,5,7-tetramethyl-8-(3-(N,N-dimethylaminomethyl)-4-hydroxyphenyl)BODIPY chloride

Availability: Maintained in stock

Appearance: Orange solid

Molecular Weight: 433.73 g.mol^-1

Stability / Storage: Store in the dark, preferably at 0-10°C. Avoid contact with moisture.

Solubility: The dye is very well soluble in alcohols (such as methanol or ethanol), acetonitrile or DMSO. The dye is also well enough soluble in aqueous solution to allow spectroscopic applications (c < 10^-4 M).

Description for Applications: Fluorescent BODIPY dye, which is photochemically stable upon excitation at 500 nm, but undergoes photochemical reaction upon excitation at 355 nm, whereupon reactive intermediate quinone methide is formed. Quinone methide reacts with proteins, so the dye can be used in photochemical labeling of proteins.

Absorption and emission spectra in acetonitrile/water (1:9)

Left figure : confocal image of live MCF-7 cells stained with the dye (c = 10^-6 M, λ_exc = 488 nm, λ_em = 500-550 nm); Right image: gel after deanturing electrophoresis; left line bovine serum albumin treated with N2 and irradiated at 350 nm; middle line, not irradiated, right line mark; upper gel stained with Comassie blue).

Biological Activity: Zlatić, K.;Antol, I.; Uzelac, L.; Mikecin Dražić, A.-M.; Kralj, M.; Bohne, C.; Basarić, N.: “Labeling of Proteines by BODIPY-Quinone Methides Utilizing Anti-Kasha Photochemistry”, ACS Appl. Mater. Interfaces 12 (2020) 347-351.

Licensing Information: Patent application: Basarić, N.; Kralj, M.; Mikecin, A.-M.; Cindrić, M., Quinone-methide precursors with BODIPY chromophore, method of preparation, biological activity and application in fluorescent labeling PCT/HR2017/000005. Granted patent in USA, UK, France and Germany.